Development and Validation of First Order Derivative Spectrophotometric Method for Estimation of Alfuzosin Hydrochloride and Finasteride in Combined Dosage Form

The present manuscript describe simple, sensitive, rapid, accurate, precise and economical first derivative spectrophotometric method for the simultaneous determination of alfuzosin hydrochloride and finasteride in combined tablet dosage form. The derivative spectrophotometric method was based on the determination of both the drugs at their respective zero crossing point (ZCP). The first order derivative spectra were obtained in methanol and the determinations were made at 258 nm for alfuzosin hydrochloride and 223 nm for finasteride. The linearity was obtained in the concentration range of 2-12 μg/ml for both alfuzosin hydrochloride and finasteride. The mean % recovery was 100.79 and 99.89 % for alfuzosin hydrochloride and finasteride respectively. The method was found to be simple, sensitive, accurate, precise and was applicable for the simultaneous determination of alfuzosin hydrochloride and finasteride in tablet dosage form. Pinal Patel* 1 , Dhara Patel 1 , Sharav Desai 2 1 Department of Quality Assurance, Pioneer Pharmacy Degree College, Nr. Ajwa crossing, Sayajipura, Vadodara-390019 2 Department of Pharmaceutical Microbiology and biotechnology, Pioneer Pharmacy Degree College, Nr. Ajwa crossing, Sayajipura, Vadodara-390019 Submission: 11 March 2015 Accepted: 21 March 2015 Published: 25 March 2015 www.ijppr.humanjournals.com Citation: Pinal Patel et al. Ijppr.Human, 2015; Vol. 2 (4): 184-193. 185